Serilesine^® - promoting better bonding of keratinocytes

Different clinical tests were done to prove that Serilesine helps to keep skin aging at bay by stimulating various mechanisms.

This page looks at how it promotes better bonding (adhesion) of keratinocytes.

To place this in context with how it helps to fight aging and to have a younger looking skin, please look at the Serilesine summary page by clicking here.

Specific keratinocyte adhesion

• For more on keratinocytes please click here.

Several microwells were coated with different concentrations of the peptide, and others were coated with BSA (Bovine Serum Albumin). Keratinocytes were added to the wells, and later washed to determine how many remained stuck to the wells.

The amount of cells stuck to BSA was taken as a baseline (non specific binding). The binding of cells to SERILESINE® was specific and took place via specialized receptors. Using 10% SERILESINE® SOLUTION in the final formula corresponds to 50 µg/ml peptide in the product, which increases cell adhesion 60%.

A microtiterplate was coated with 50 µl per well of the product SERILESINE® (0.5 - 250 µg/ml per well) in distilled water, by drying overnight at room temperature.

Control wells to evaluate the non-specific cell adhesion were coated with 40 µM bovine serum albumin (BSA). All wells were then washed with 200 µl PBS and blocked for 1h with 1% BSA in PBS at 37ºC.

Human Epidermal Keratinocytes were trypsinised, labeled for 30 min at 37ºC in 5 µM Calcein-AM (Molecular Probes) and washed three times in Epilife medium. Labeled cells (4.5 x104 /well) were added to the previously coated plate and incubated for 2h at 37ºC in 5% CO2 humidified air. Cell adhesion was assessed by measurement of calcein fluorescence before and after washing non-adhered cells.

Percentage of adhesion was determined by dividing the fluorescence of adhered cells by the total fluorescence of cells added to each well. Cell adhesion promoted by the product was calculated in respect of non-specific cell adhesion to BSA.

Results show that the product SERILESINE® is able to promote keratinocyte adhesion in a dose dependent manner.

The non-specific cell binding was taken as a baseline and the specific cell attachment to SERILESINE® was calculated as the relative excess percentage (compared to non-specific attachment). At the highest concentration of SERILESINE® (250 µg/ml) we found 65% more cells linked to the hexapeptide than cells linked to non-specific substrates (plastic and BSA).

These results are indicative that SERILESINE® is recognized by specialized receptors on the surface of keratinocytes.

Due to the fact that combining all the images on one page makes it too large to download within a fair period of time, we created different pages dealing with each aspect that was tested.

The other test that were performed can be viewed by clicking the hyperlink.

• Serilesine increases Laminin (I)
• Serilesine increases Laminin (II)
• Serilesine increases a6-Integrin production
• Serilesine stimulates hemidesmosome formation
• Serilesine increases keratinocyte bonding
• Serilesine increases keratinocyte creation
• Serilesine increase the creation of fibroblast
• Serilesine increases skin elasticity
• Serilesine increases skin compactness (firmness)
• Serilesine increases skin tone
• Serilesine increases skin smoothness

If you are looking for the summary page of Serilesine please click here.